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Introduction (see below)
Mushroom Body Complex
Central Body Complex
Receptor Terminals in progress
Mechanosensory Neuropils in progress
Antennal Nerve Endings
Protocerebral Neuropils (except Central and Mushroom Body Complexes)
Thoracic-Abdominal Ganglia in progress
Staining Method (see below)
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Images were collected using a Leitz Diaplan microscope equipped with a Sony DKC-5000 CCD with image-capture software, running as a plug-in through Adobe Photoshop. Each image was scanned at 200 dpi, using a 40x or 100x oil-immersion planapochromat objective with either 1x or 0.6x intermediate magnification. Many of the Golgi and cobalt pictures are composite images. They have been reconstructed from serial optical sections taken at every 2 µm of depth through 1-4 consecutive 12-14 µm thick plastic sections. Initial alignment was manual: outlines of the last optical section of the first plastic section were drawn on a transparent overlay on the monitor, and the first optical section of the second section was aligned to this rendition using a rotating stage and X Y controls. Final alignment was accomplished using software. Each captured image was defined as a separate layer, and successively overlaid with the next captured image using the software compositing controls on 'DARKEN'. Due to the high contrast of the histological material, namely, dark staining on a light background, the most highly defined structures at each depth of focus are additively displayed. The images were then saved and labeled in CorelDraw (approx. 25MB), and reduced to JPEG format (approx. 150KB). Examples of this technique can be seen by clicking here or here.
Other images, such as those submitted by contributors, were digitized from transparencies using a Hewlitt Packard Scanjet 3C/T and scanning hardware. The resulting images were labeled using CorelDraw. Compression reduced the file size from TIFF (approx. 5MB) to JPEG (approx. 150 KB).
Strausfeld, N.J. (1980) The Golgi method: Its application to the insect nervous system and the phenomenon of stochastic impregnation. In: Strausfeld, N.J., Miller, T.A. (Eds.) Neuroanatomical Techniques. Insect Nervous System. Springer. New York. pp 132-205.
|Flybrain Atlas Golgi Sections|
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AA00032Page last modified: July 25, 2000 by Managers.