Mushroom Body is a Quadruple Structure

Mushroom Body is a Quadruple Structure - Figure 4

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19 GAL4 enhancer-trap strains reveal overlapping cell types in all four of the MBNb lineages.

We screened 488 GAL4 enhancer-trap strains for staining in the MB, and found 19 strains that label various subsets of the MB cells. Surprisingly, all 19 strains revealed four clusters of cells in the MB cortex and four bundles of fascicles at the root of the pedunculus. This strongly suggests that the sets of cells in each of the four clusters are essentially identical.

Click each thumbnail image to see the original figure.

The GAL4 expression was detected using UAS-tau. Rows 1 and 2 and the left half of row 3 show the strains that label neurones that project to the medial bundle of the pedunculus. The right half of row 3, row 4, and the left half of row 5 show the strains that label a larger number of neurones in both medial and lateral bundles of the pedunculus. Most strains show clear separation between the clusters. The border between the clusters is less clear in Mz 1045 of row 5, which labels only a few scattered neurones that innervate the lateral bundle and the gamma lobe. In several enhancer-trap lines (i.e. Np 35, Mz 918, Mz 1081, Mz 1162 and Mz 529 of Fig. 4), two of the four fascicles in the pedunculus medial bundle fuse first as they project from posterior to anterior, forming two bundles that eventually fuse at a more anterior position.

All figures are montaged from 10-30 photographs. Bar, 50 um (10-um scale).

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Flybrain Development Mushroom Body Quadruple Structure Quadruple Structure - Figure 4

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