Mushroom Body is a Quadruple Structure - Figure 5

Fig. 5. Spacial relationship between the four fascicles, clusters and glial cells.

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Np 178

Mz 1530

GAL4 strains Np 178 (A) and Mz 1530 (B) were crossed to UAS-GFP S65T (green). Four clusters of cell bodies (labelled with numbers 1 to 4) as well as four bundles of fascicles (numbers 1 to 4 in italics) running towards the pedunculus are observed. Although there is no twist between the neighbouring fascicles, the alignment of the four fascicles and cell body clusters is not identical in different individuals.

Glial cells (blue, labelled with the glial-specific anti-Repo antibody) are observed both in the cell body clusters and between the four fascicles. Of the two classes of these glial cells, only the former are labelled in the flippase-mediated clones (see Fig. 3F).

Three-dimensional reconstructions were made from about 200 confocal 0.4-um optical sections. Bar, 10 um.

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