GAL4-Responsive UAS-tau as a Tool
for Studying the Anatomy and Development of the
Drosophila Central Nervous System

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Kei Ito 1,3, Heinz Sass 1,4, Joachim Urban 1, Alois Hofbauer 2 and Stephan Schneuwly 2
1 Institut fuer Genetik, Universitaet Mainz, Saarstr. 21, D-55122 Mainz, Germany
2 Lehrstuhl fuer Biologie VI, Institut füer Zoologie, Universitaet Regensburg, Universitaetsstr. 31, D-93040 Regensburg, Germany
3 present address to whom the correspondence should be addressed: Nishibonaka 38, Myodaliji, Okazaki 444-8585 Aichi, Japan
4 present address: Institut füer Mikrobiologie und Genetik, Universitaet Leipzig, Johannisallee 21, D-04103 Leipzig, Germany

Originally published in:
Kei Ito, et al., 1997, GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system, Cell and Tissue Research 290:1-10


To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive marker UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.

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Fig. 1:
Mechanism of the GAL4 enhancer trap with UAS-tau
Fig. 2:
The T1 cell in the adult Drosophila CNS (C850)
Fig. 3:
Three-dimensional reconstruction of the Tau-labelled cells (Mz 699 & Mz 423)
Fig. 4:
Formation of the median bundle and mACT (Mz 699)
Fig. 5:
Development of the DGI (Mz 423)


These pages show only the original figures (and the legends for them) that appeared in the article shown above. Due to the copyright issue, the full text is not available here.

Go to Index | Fig. 1 | Fig. 2 | Fig. 3 | Fig. 4 | Fig. 5

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