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Central Complex Architectures

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The structure of neuropil can be revealed by a variety of complementary methods. In this module, central body neuropils are revealed by three different techniques: cobalt uptake, the Bodian reduced silver method and immunohistology.


Figure 1.

Fig. 1. A-C. Three levels of focus through the brain of Calliphora erythrocephala showing neurons originating above the protocerebral bridge, with dendrites in the bridge, sending axons to the central body. The axons arborize in the superior arch (B) and then project into the upper half of the ellipsoid body (C). This level also shows the bipartite division of the noduli with axons extending to the ventral bodies. These symmetrical patterns of cell staining were achieved by focal injection of cobalt into the right ventral body. D-G. Consecutive Bodian-stained sections through a brain of Pheanicia sericata from the protocerebral bridge through the fan shaped body. This series is from a brain tilted slightly such that the posterior protocerebral bridge was in the same plane of section as the noduli. The width of the protocerebral bridge (Fig. 1A, D) is approximately 100 µm. The depth of the fan shaped body (Fig 1F, G; Fig 2B), at the mid-line, is approximately 100 µm.


Figure 2.

Fig. 2A, B Central body region showing anti-tyrosine hydroxylase labeling (dopamine containing neurons) revealed by peroxidase/anti-peroxidase secondary antibody labeling compared to anti-FMRFamide labeling (green) and anti-leucokinin labeling (red).


Figure 3.

Fig. 3A. Vertical section through the brain of Phormia stained with antibodies raised against FMRFamide, secondary antibody labelled with FITC (green), and with antibodies against leucokinin, amplified by secondary antibody labelled with TRITC (red). Note the labeling in the noduli, showing the outer shell of immunoreactivity corresponding to similarly disposed processes seen in Golgi preparations of Drosophila. Note also thelarge immunofluorescent cell bodies in the pars intercerebralis. Fig. 3B. Same level showing distribution of anti-leucokinin (TRITC-labelling; green) and anti-SCPB (AMCA labeling: blue).


Figure 4.

Fig. 4A, B. compares fan-shaped body and superior arch stained with antibodies against leucokinin and SCPB with the same level stained with antibodies raised against myomodulin (TRITC; red) and SCPB (blue).

Immunocytological preparations: Dr. D.R. Nässel, Stockholm University. Cobalt and silver preparations: Dr. N.J. Strausfeld, University of Arizona.


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