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Central Complex Architectures
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The structure of neuropil can be revealed by a variety of complementary
methods. In this module, central body neuropils are revealed by three
different techniques: cobalt uptake, the Bodian reduced silver method and
immunohistology.
Figure 1.
Fig. 1. A-C. Three levels of focus through the brain of Calliphora
erythrocephala showing neurons originating above the protocerebral bridge,
with dendrites in the bridge, sending axons to the central body. The axons
arborize in the superior arch (B) and then project into the upper half of
the ellipsoid body (C). This level also shows the bipartite division of the
noduli with axons extending to the ventral bodies. These symmetrical
patterns of cell staining were achieved by focal injection of cobalt into
the right ventral body. D-G. Consecutive Bodian-stained sections through a
brain of Pheanicia sericata from the protocerebral bridge through the fan
shaped body. This series is from a brain tilted slightly such that the
posterior protocerebral bridge was in the same plane of section as the
noduli. The width of the protocerebral bridge (Fig. 1A, D) is approximately 100 µm. The depth of the fan shaped body (Fig 1F, G; Fig 2B), at the mid-line, is approximately 100 µm.
Figure 2.
Fig. 2A, B Central body region showing anti-tyrosine hydroxylase labeling (dopamine
containing neurons) revealed by peroxidase/anti-peroxidase secondary
antibody labeling compared to anti-FMRFamide labeling (green) and anti-leucokinin
labeling (red).
Figure 3.
Fig. 3A. Vertical section through the brain of Phormia stained with
antibodies raised against FMRFamide, secondary antibody labelled with FITC
(green), and with antibodies against leucokinin, amplified by secondary
antibody labelled with TRITC (red). Note the labeling in the noduli,
showing the outer shell of immunoreactivity corresponding to similarly
disposed processes seen in Golgi preparations of Drosophila. Note also thelarge immunofluorescent cell bodies in the pars intercerebralis.
Fig. 3B. Same level showing distribution of anti-leucokinin
(TRITC-labelling; green) and anti-SCPB (AMCA labeling: blue).
Figure 4.
Fig. 4A, B. compares fan-shaped body and superior arch stained with
antibodies against leucokinin and SCPB with the same level stained with
antibodies raised against myomodulin (TRITC; red) and SCPB (blue).
Immunocytological preparations: Dr. D.R. Nässel, Stockholm University.
Cobalt and silver preparations: Dr. N.J. Strausfeld, University of Arizona.
Atlas