Optic lobe development: GFP expression driven by Gal4(Mz1369) counterstained with mab22C10

Three confocal stacks of larval brain hemispheres of Mz1369/UAS-gfp, counterstained with mab22C10, in sagittal, horizontal and frontal orientation provide insights into the structure of the developing optic lobe in the larval stage. Mz1369 targets strong Gal4 expression to the inner optic anlage (IOA), a complex epithelial structure that proliferates new neurons contributing to the lobula complex and proximal medulla during larval and early pupal development. The IOA is difficult to visualise with conventional histological methods. These datasets in three orthogonal orientations supply a reference for structures in the developing OL and are the base for three-dimensional reconstructions of larval OL structure.

Resources:

1. Projections from individual stacks

These are in full 512x512 resolution. Size of individual files is 30-40K (Previews in 128x128/4 K each). Raw as well as annotated versions are available.

Sagittal
Horizontal
Frontal


2. The three stacks in sagittal, horizontal and frontal orientation

The original stacks are 512x512 pixels with 107 to 146 images depth. The movie files on the following pages are reduced by a factor of two in all dimensions (file size 1 - 1.6 M).

Page with all stacks as embedded QT movies
Sagittal stack
Horizontal stack
Frontal stack



3. Hemisphere Browser

   This is a frame based page that lets the viewer select from the projection data sets of all orientations and browse through them, while simultaneously comparing the projections with selectable movie files of all orientations. Annotations can be toggled on and off, and 3D reconstructions can be accessed.

4. Three dimensional reconstructions

VRML 1 model with silhouette of hemisphere
(requires VRML viewer capable of displaying transparency)
VRML 1 model without hemisphere.
VRML 2 model with hyperlinked annotations
(requires frames-enabled browser, VRML 2 viewer).
Hemisphere Browser to view VRML 1 files together with stacks.