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Optic Lobe Development
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Five projections, proceeding from distal to proximal in the hemisphere,
were made with the extended focus algorithm of Bitplane Imaris. Each
image contains information from 10-15 optical sections.
The images are a combination of maximal value and hardware blended overlays.
Anterior is to the left and posterior to the right in this series.
Red is mab22C10 fluorescence and green is GFP-fluorescence.
Abbreviations used in annotations: os, optic stalk; ioa, inner optic anlage; ed, eye disc; t&c, T&C cell group; ioc, inner optic chiasm; ooc, outer chiasm; mc, medulla cortex; lc, lobula complex; lpc, lobula plate cortex; tmn, transient medulla neuropil; bn, bolwig nerve; cbf, cell body fibres.
Raw version |
annotated version
Most distal projection. Entrance of optic stalk into brain.
Raw version |
annotated version
Arc of T&C cell projections is clearly visible. A part of the IOA reaches to
the surface of the hemisphere.
The horseshoe-shaped group of undifferentiated cells between the T&C
cell projections and the lobula plate cortex area is derived from the IOA
and connected to it with strands of cells which are visible in stacks of different orientation.
Raw version |
annotated version
The outer chiasm and the projection of the T&C cells into the inner chiasm can be followed in this image.
Raw version |
annotated version
View into the inner optic chiasm.
Strands of cells proliferated from the inner optic anlage are interlaced with the projections connecting
medulla and lobula complex.
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annotated version
Most proximal projection. Inner horseshoe shape of IOA.
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