Figure 2 - Expression of Nuclear Regulatory Genes in the Embryonic MB Primordia

Figure 2 - Expression of Nuclear Regulatory Genes
in the Embryonic MB Primordia

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Figure 2
Expression of Nuclear Regulatory Genes in the Embryonic MB Primordia.
Laser confocal microscopy of embryonic brains at stage 16 (A) and stage 15 (B-F). Embryonic brain was visualized with neuron-specific anti-HRP antibody (green) as described (ref. 12, 13). (A, B) Expression of an embryonic MB marker, 238Y(blue; ref. 7), and EY (red; ref. 2) in the embryonic brain. Dorsal view of whole brain (A) and lateral view of the MB primordium at higher magnification (B). Note coexpression (pink/violet) of EY and 238Y. 238Y expression is particularly strong in two of the MB neuroblasts and their progenies. Arrows, MB primordia. Stars, MB neuroblasts. Scale bar, 10 µm (A) and 25 µm (B). (C) Expression of toy (ref. 3) transcripts (yellow) in the embryonic MB. Fluorescent in situ hybridization (ref. 14). Lateral view of the MB primordium at higher magnification. Stars, MB neuroblasts. (D) Expression of EY (blue) and DAC (red; ref. 4) in the embryonic brain. Note the coexpression (pink/violet) of EY and DAC in the MB primordia (arrows). (E) Expression of Eyes absent (EYA, red; ref. 15) and 238Y (blue) in the embryonic brain. EYA is expressed in nearby cells but not in the MB primordia (arrows). (F) Expression of sine oculis (so; refs. 16, 17) transcripts (yellow) in the embryonic MB. Fluorescent in situ hybridization. Lateral view of the MB primordium at higher magnification. Stars, MB neuroblasts.

Flybrain Development Genetic Control of MB Development Figure 2

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