|
Figure 3 - Expression of Nuclear Regulatory Genes
in the Larval MBs.
|
Figure 3
Expression of Nuclear Regulatory Genes in the Larval MBs.
Laser confocal microscopy of third instar larval brains. Reconstruction of
optical sections (A) and single optical sections (B-I). (A) Larval MB labelled
for a Gal 4 MB marker, 201Y (green; ref. 18), and EY (red). Lateral view
showing the Kenyon cells (KCs), calyx (cx), peduncle (ped), and the larval lobes
(alphaL and betaL). Note prominent coexpression (yellow) of EY and 201Y
in Kenyon cells that locate above the calyx. Bar, 50 µm. (B, C) Expression of EY
(red/yellow) and 201Y (green/yellow) in larval Kenyon cells. Dorsal views. (B)
EY expression, (C) same as B but shows both EY and 201Y. Star, MB neuroblasts.
Bar, 40 µm. (D) Expression of toy in Kenyon cells. Fluorescent in
situ hybridization. Dorsal view. Identical pattern was confirmed with anti-TOY
antibody (U. W. unpublished data). (E, F) Differential expression of EY
(green/yellow) and DAC (red/yellow) in the larval Kenyon cells around the four
MB- neuroblasts (stars). (E) EY expression, (F) same as E but shows both EY
and DAC. (G, H) Differential expression of DAC (red/yellow) and Gal4 MB
marker c831 (green/yellow; ref. 18) in larval Kenyon cells. (G) DAC expression,
(H) same as G but shows both DAC and c831 (Yang et al., 1995). (I) Expression
of EY (green) and so-lacZ (red; ref. 15) in larval brain. Similar result
was obtained by in situ hybridization for so transcripts.
Table 1: Summary of the expression of Pax6 and other regulatory genes in the larval MBs.
| |
ey |
toy |
dac |
eya |
so |
MB-Gal4 |
|
NBs |
+ |
+ |
- |
- |
- |
- |
|
GMCs |
++ |
++ |
+/- |
- |
- |
- |
|
Inner KCs |
++ |
++ |
++ |
- |
- |
- |
|
Outer KCs |
++ |
++ |
++ |
- |
- |
++ |
NBs, neuroblasts; GMCs, ganglion mother cells; KCs Kenyon
cells. MB-Gal4 includes 201Y, c831, and 238Y, which show identical
patterns in the larval Kenyon cells.
Development