Figure 2 - Coexpression of the EXD and HTH Proteins with Neuraxial Patterning Genes

Laser confocal microscopy of stage 14 embryos. Brain is labeled with anti-HRP (green). Reconstruction of optical sections. Bar, 20 µm (B) Same preparation as in A but shows both ot transcripts (blue) and the EXD protein (red). Arrows, otd cell clusters in neuromere b1; arrowheads, dorsal otd cluster in neuromere b2. See Hirth et al. (1995) for neuromeric organization of the embryonic brain. (C) Expression of the EMS protein (blue). (D) Same preparation as in C but shows both EMS (blue/pink) and EXD (orange/pink) proteins. Arrows, EMS expression in the anterior part of neuromere b2; arrowheads, EMS expression in the anterior part of neuromere b3. (E) Expression of lab (blue) in the posterior part of neuromere b3 as revealed with a lab-lacZ reporter (Chouinard and Kaufman, 1991). (F) Same preparation as in E but shows both lab-lacZ (blue/pink) and EXD (orange/pink) proteins. Arrows, lab-lacZ expression in the central nervous system. (G) Expression of the DFD protein (blue; Regulski et al., 1987) in the mandibular neuromere and the anterior half of the maxillary neuromere. (H) Same preparation as in G but shows both DFD (blue/pink) and EXD (orange/pink) proteins. Arrows, DFD expression in the central nervous system. (I) Expression of the SCR protein (blue; LeMotte et al., 1989) in the posterior half of the maxillary neuromere and the anterior half of the labial neuromere. (J) Same preparation as in I but shows both SCR (blue/pink) and hth (orange/pink) as revealed with a hth-lacZ reporter (Salzberg et al., 1997). Arrows, SCR expression in the central nervous system.

Flybrain    Development    Patterning Defects    Figure 2

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