Figure 4 - Expression of Neuraxial Patterning Genes in exd Mutant Brain

Bar, 20 µm;m. Laser confocal microscopy of wild type (A, C, E, G, I) and maternal and zygotic exd null embryos (B, D, F, H, J). Reconstruction of optical sections. Stage 14. Brain is labeled with anti-HRP antibody (green). (A, B, C, D) Expression of the otd gene in the developing brain. Embryos are labeled with specific otd RNA probe (yellow/red). Arrows, otd cell clusters in neuromere b1; arrowheads, dorsal otd expressing cells in neuromere b2; open arrows, otd expression in neuromere b3. (A) Dorsal image of wild type brain. Note the clear anterior and posterior boundaries of the b1 otd domain. Brain commissure is not included in this reconstruction. (C) Lateral image of wild type brain. The otd expression in neuromere b3 is obscured in this reconstructed image by the strong esophagus signal. es, esophagus. (B) Dorsal image of exd mutant brain. The brain commissure (bc) is abnormally shifted posterior (in neuraxis). ep, epidermal otd expression. (D) Lateral image of exd mutant brain. Note the expansion of otd expression in many parts of the brain including the dorsal region. (E, F) Expression of the EMS protein (yellow/red). Arrow, EMS expression in the anterior part of neuromere b2; arrowhead, EMS expression in the anterior part of neuromere b3. (G, H) Expression of the LAB protein (red). Arrow, LAB expression in the posterior part of neuromere b3. (I, J) Expression of the DFD (red) and SCR (blue) proteins. sg: salivary gland, which is lost in exdmutant.

Flybrain    Development    Patterning Defects    Figure 4

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