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Figure 5 - Expression of EY in Wild Type and exd Mutant Brains |
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Bar, 20 µm. Reconstruction of optical sections. Brain is visualized with anti-HRP antibody (green). (A, D) Wild type embryos at stage 14, labeled with anti-EY (orange/yellow; U. W., unpublished) and anti-EMS (blue) antibodies. Lateral view (A) and dorsal view (D). White arrows, EY expression in neuromere b1; white arrowheads, EY expression in the anterior part of neuromere b2; open arrows, EY expression in the anterior part of neuromere b3. Yellow arrows, EMS expression in the posterior part of neuromere b2; yellow arrowheads, EMS expression in the posterior part of neuromere b3. Note the distinct molecular subdivisions of the developing brain neuromeres. (B, E) Wild type embryos at stage 16, labeled with anti-EY antibody (orange/yellow). Lateral view (B) and dorsal view (E). Arrows, EY expression in neuromere b1; arrowheads, EY expression in neuromere b2; open arrows, residual EY expression in neuromere b3. (C, F) maternal and zygotic exd null embryos at stage 16, labeled with anti-EY antibody (orange/yellow). Arrows, remaining EY clusters at the anterior terminal region of the brain.
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