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Three-dimensional Reconstruction of the Drosophila Larval and Adult Brain

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Karlheinz Rein1*, Peter Robin Hiesinger2*, Malte Zöckler3, Jan Kirsten1, Karl-Friedrich Fischbach2, and Martin Heisenberg1

*The first two authors contributed equally to this work

1University of Würzburg, Lehrstuhl für Genetik, Biozentrum, Am
Hubland, 97074 Wuerzburg, rein@biozentrum.uni-wuerzburg.de

2University of Freiburg, Institute for Biology III, Schaenzlestr. 1, D-79104
Freiburg, Germany, robin@biologie.uni-freiburg.de

3Konrad-Zuse-Zentrum für Informationstechnik, Takustr. 7, D-14195
Berlin-Dahlem, zoeckler@zib.de


Overview

Here we present reconstructions of individual wild type Drosophila larval and adult brains. To obtain these, whole brains were dissected from heads and labeled fluorescently. Using a confocal microscope we obtained about 200 optical sections (thickness about 1 Ám) for each brain. The individual brain structures in these sections were labeled manually and surfaces were generated (see also Rein et al., 1999). We brought the surfaces and parts of the original image data into the VRML format, so that they can be viewed on normal desktop computers using a web-browser and a freely available plug-in. Movies showing the brains from different orientations were generated using volume visualization techniques.



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