Figure 2 - Confocal Microscopy of Adult Brains

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(A) Line c161 stained cell bodies clustered in the medial protocerebrum which send projections to the glomeruli of the pb; axons also arborize throughout the eb and both the dorsal (dno) and ventral (vno) regions of the no. This pattern is highly reminiscent of pb-eb-no neurons described by Hanesch et al. (1989). There is also expression in a network of fibers just dorsal to the anterior medial protocerebrum that appears to be connected to the cc neurons. (B) Line 78Y stained a cluster of cc neurons located in a more lateral position than that of line c161 (some overlap cannot be discounted). They project to the pb and from there progress to the eb, where they innervate the inner/midregion of the ring from the canal outward. The no staining in line 78Y is restricted to the dorsal regions (dno), and in Figure 2 (E) was obscured by eb staining. Scale bar = 50 µm.

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