Figure 5 - Enhancer-trap Patterns

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These examples illustrate whole-mount confocal microscopy of adult brains double labeled for different enhancer-trap expression patterns. (A,C) Frontal perspectives that represent reconstructions of ~ 50 µm thickness. (B,D) Approximately 5-µm cross-sections reconstructioned from (A,C), respectively, such that anterior is to the top of the figure. (A) c105 axons (green) mark the inner zone of the eb, while pwF22-6 axons (red) mark its outer zone, consistent with the R1 and R4 designations, respectively. (B) In cross-section, R4 neurons are restricted to the anterior layer, while R1 neurons arborize in the posterior layer. (C) 189y axons (green) occupy inner and medial zones of the eb and do not overlap with R4d axons (red) in the outer zone. (D) In cross-section, the majority of the 189y-labeled axons arborize in the anterior layer of the eb consistent with an R3 designation; some, however, also extend to the posterior layer (D). The 189y axons (D) do not progress as far posterior as do c105 axons (B). For all images, the scale bar = 20 µm.

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