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(A-C) Single optical sections showing the embryonic MB tracts. Lateral views. The embryonic MB tracts are labeled with UAS-tau-lacZ (green) driven by 238Y-GAL4. The major brain tracts are visualized with anti-FAS II antibody (magenta).

(A) Stage 14. Arrowheads indicate an interface cell that co-expresses FAS II and 238Y on its surface. MB neuroblasts are indicated with stars.

(B) Stage 15. The 238Y MB neurons extend thin pioneer axons (arrows) along the FAS II-expressing cells.

(C) Stage 16. The MB tracts converge at LPT. FAS II expression on the nearby cells is down regulated by this stage while the growing MB tracts (arrows) become more prominent.

(D,E) Late stage 16. Reconstructed from optical sections.

Lateral (D) and dorsal (E) views. The 238Y signal (green) is selectively enhanced to show the embryonic MBs. Major FAS II tracts also are shown (white). The embryonic brain hemisphere and its core neuropil are outlined with brown line. ACT, antennocerebral tract; BN, Bolwig’s nerve; DCT, dorsal commissural tract; fc, frontal commissure; LPT, lateral protocerebral tract; MPT, medial protocerebral tract; OL, optic lobe; P1, P4l, P4m, P5l, fiber tract founder clusters (Nassif et al., 1998) Ped, peduncle; VCT, ventral commissural tract. Scale bars: in A, 10 mm for A-C; 10 mm in D,E.

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