Differential expression of MB markers in the first instar brain.

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(A,D,G,J) Expression of dnc-lacZ is revealed by an anti-b-GAL antibody (green/white). (B,E,H,K) GFP expression driven by 201Y (green/white). (C,F,I,L) GFP expression driven by c739 (green/white). (A-C) Slightly oblique dorsal views of MBs showing the Kenyon cells labeled with an anti-DAC antibody (magenta/white). Stars indicate the positions of the MB neuroblasts. (D-F) Lateral views showing the peduncle and lobes. Major axonal tracts, lobes and peduncle are labeled with an anti-FAS II antibody (magenta/white). Note that FAS II is expressed in the lobes and the distal part of the peduncle. (G-I) Single optical sections of the medial lobes. Marker expression is shown on the right in single channel. (J-L) Single optical sections of the dorsal lobes. Marker and FAS II expressions are shown on the right in single channels. Similar internal staining was obtained for peduncle sections (not shown). Scale bars: in A, 20 mm for A-C; in D, 20 mm for D-F. ACT, antennocerebral tract; cx, calyx; DL, dorsal lobe; h, heel; ML, medial lobe; Ped, peduncle.

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