Differential expression of MB markers in the second instar brain.

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(A,D,G,J) Expression of dnc-lacZ (green/white). (B,E,H,K) GFP expression driven by 201Y (green/white). (C,F,I,L) GFP expression driven by c739 (green/white). (A-C) Dorsal views of the Kenyon cell clusters labeled with an anti-DAC antibody (magenta/white). Stars indicate the positions of the MB neuroblasts. (D-F) Lateral views. The peduncles and lobes are labeled with an anti-FAS II antibody (magenta/white). (G-I) Single optical sections of the peduncles and the medial lobes. Marker expression is shown on the right in single channel. (J-L) Single optical sections of the dorsal lobes. Marker and FAS II expressions are shown on the right in single channels. Note similar internal staining for the peduncles and the lobes. Scale bars: in A, 25 mm for A-C; in D, 25 mm for D-F. ACT, antennocerebral tract; cx, calyx; DL, dorsal lobe; h, heel; ML, medial lobe; Ped, peduncle.

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