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(A) Lateral view of wandering third instar MB labeled with an anti-FAS II antibody (magenta/white) and phalloidin (green/white).

(B,C) MB axonal fibers around the calyces. MBs are labeled with UASmCD8:: GFP (green/white) driven by 201Y and phalloidin (magenta/white). Single optical sections of wandering third instar MBs.

(D-I) GFP reporter staining in wandering third instar MBs. GAL4 drivers are (D-F) elav-GAL4, (G,H) OK107 and (I) 201Y. OK107 is expressed moderately in the MB neuroblasts and GMC, and strongly in the differentiated Kenyon cells.

The reporters are (D,F-I) UAS-mCD8::GFP (green/white) and (E) UAS-GFP (green/white).

(D,G) Dorsal views of Kenyon cells labeled with anti-DAC antibody (magenta); stars indicate the positions of the neuroblasts.

(E,F,H,I) Lateral views of the lobes and the distal part of the peduncle labeled with anti- FAS II antibody. Note the core staining with the elav-GAL4 and OK107 drivers. Insets are cross sections of peduncles. Scale bars: in A, 20 mm for A,E,F,H,I; 20 mm in B,C; in D, 20 mm for D,G. ACT, antennocerebral tract; cx, calyx; DL, dorsal lobe; h, heel; ML, medial lobe; Ped, peduncle.

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