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(A) Axonal projections of embryonic MB primordium in fas IIeb112 mutant (protein null). Lateral view of the embryonic brain at late stage 16. FAS II (magenta) and 238Y (green). Single optical section. The embryonic MBs are labeled with UAS-tau-lacZ and 238Y-GAL4. Arrows indicate the growing MB axons.

(B) Overview of an embryonic MB primordium in fas IIeb112. Reconstruction of optical sections. Same embryo as in A. Neurons are labeled with anti-HRP antibody (white). 238Y signal (green) is selectively enhanced.

(C,D) Third instar larval MBs double labeled with anti-FAS II (magenta/white) and anti-DIF (green/white), which stains MB structures (Cantera et al., 1999).

(C) fas IIe76 hypomorphic (10%) mutant. Note the thin dorsal lobe (arrowhead) and fusion of the medial lobes.

(D) fas IIe86 hypomorphic (50%) mutant. Note the small dorsal lobe (arrowhead) and the medial lobes (arrow). Calyces are markedly expanded with aberrant accumulation of the FAS II protein.

(E,F) fas IIeB112 mutant clones at the late third instar stage (green/white). Mitotic recombination was induced in the first instar stage.

(E) Dorsal view of the Kenyon cells labeled with anti-EY (blue). Stars indicate neuroblasts.

(F) Lateral view showing the peduncle, dorsal and medial lobes labeled with anti-FAS II (magenta/white). Inset shows a cross section of the peduncle. The core fibers are not labeled owing to the driver/reporter combination used for the generation of the mosaic clones. Genotype used: hs-FLP, tubP-GAL80, FRT19A/ fas IIeB112, FRT19A; 201Y/ UAS-GFP-T2. cx, calyx; DL, dorsal lobe; ML, medial lobe; Ped, peduncle. Scale bars: 10 mm in A,B; in C, 50 mm for C,D; 20 mm in E,F.

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