Commissure Formation

Figure 2
Commissure formation. Frontal views through the brain hemispheres (laser confocal microscopy).

Neuron-specififc anti-HRP immunoreactivity (A,D,E,F,G), glial cell-specific repo immunoreactivity (H), combined anti-HRP immunoreactivity (red) and repo immunoreactivity (green/yellow) (B,C,I).

(A,C) Cellular protrusions (A, Arrows) composed of neurons and glial cells extend from median edges of hemispheres towards midline. Small group of neurons and glial cells at midline (open arow).(A) Reconstruction of brain hemispheres,
(B,C) different optical sections.
(D-F) Establishment of initial commissural axonal projection (D,F, arrows) in close association with interhemispheric cell bridge (E, arrowhead). (D) Reconstruction of brain hemispheres,
(E,F) different optical sections.
(G,I) Differentiation of commissural pathway leads to formation of preoral brain commissure (G, arrow); a row of glial cells extending across midline (H, arrowhead) is closely associated with commissure.
Stages 12/0 (A-C), 13 (D-F), 16 (G-I). Scale bar, 10.

Flybrain    Development    Embryonic Brain Development    Formation of Commissural and Descending Pathways    Commissure Formation

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