Formation of Circumesophageal Axon Pathways

Figure 3
Formation of circumesophageal axon pathways.

(A-C) Sections, (D-L) lateral optical sections in plane of a circumesophageal connective (laser confocal microscopy): Neuron-specific anti-HRP immunoreactivity (A,D,G,J), P[repo4/lacZ] revealed with anti-ß-galactosidase (B,C), glial cell-specific repo immunoreactivity (E,H,K), combined anti-HRP immunoreactivity (red) nad repo immunoreactivity (green/yellow) (F,I,L).

(A) Ascending and descending axonal processes (arrows) growing around ingrowing foregut (arrowhead); lateral section.
(B,C) Rows of longitudinal glial cells (arrowheads) extend from brain hemispheres around foregut to ventral nerve cord; frontal sections.
(D-F) Neurons of brain and subesophageal ganglion are initially separated by a distinct gap (D, arrow); this gap is bridged by a row of glial cells (E, arowhead).
(G-I) Subsequently, a neuronal cell bridge (G, arrow) is formed across the gap in close association with a row of glial cells (H, arrowhead).
(J-L) The first axonal pathways (J, arrow) are established between the brain and the subesophageal ganglion across the neuronal cell bridge and in close association with the longitudinal glial cells (L, arrowhead)
Stages 11 (D-F), 12/5 (G-I), 12/3 (A), 12/0 (J-L) and 13 (B,C). (Note that stage 12/5 preceedes stage 12/3 which preceedes stage 12/0; see Klämbt et al., 1991.) Scale bar, 10.

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