Commissure Differentiation is Perturbed in Commissureless Mutants

Figure 6
Commissure differentiation is perturbed in commissureless mutants

(A-E) Frontal view through brain hemispheres (laser confocal microscopy).
(F,G) Sections.
Neuron-specific anti-HRP immunoreactivity (A), combined anti-HRP immunoreactivity (red) and glial cell-specific rep immunoreactivity (green/yellow) (B,C), combined axon-specific BP102 immunoreactivity (red) and repo immunoractivity (green/yellow) (D), BP102 immunoreactivity (E,G), Fasciclin II immunoreactivity (F).

(A-C) An initial commissural axon pathway (A, arrow) is established along the midline cellular bridge (B, arrowheads) composed of neurons and a small number of glial cells. (A) reconstruction of hemispheres, (B,C) different optical sections.
(D,E) Subsequent differentiation of the commissural pathway is pertubed and only a single fascicle of commissural axons (E, arrows) is formed. Associated glial cells (D, arrowheads) spanning the midline appear normal.
(F) Commissural fascicle contains Fasciclin II-expressing cells. Frontal section.
(G) Mutant preoral commissure (filled arrow) is highly reduced and all postoral commissures are missing (open arrow); descending longitudinal tracts (arrow heads) appear normal. Frontal section in plane of both circumesophageal connectives.
Stages 13 (A,B,C), 14 (D,E), 16 (F,G). Scale bar 10.

Flybrain    Development    Embryonic Brain Development    Formation of Commissural and Descending Pathways    Commissure Differentiation is Perturbed

All contents are copyright © 1995-2000 Flybrain or their original publication (as noted).
Copyright and use policy

AB00107