Expression of Engrailed in the Embryonic Brain

Laser confocal microscopy of wild-type stage 12 (A-D) and stage 15 (E and F) embryos double labeled with anti-en antibody (yellow/green) and neuron-specific anti-HRP antibody (red/orange; A, B, D, and F) or labeled with neuron-specific anti-HRP antibody (red/orange; C and E). Images are reconstructions of optical sections.

(A) The two most anterior stripes of en-expressing cells are visible in this frontal view of the embryonic brain. The en-b1 stripe is labeled with arrows, the en-b2 stripe with arrowheads. The en-b3 stripe is not visible in this focal plane.
(B) the posterior stripe of en-expressing cells in the embryonic brain, en-b3, is indicated with open arrows. Sections were taken at a different focal plane than in (A).
(C) Lateral view of an embryonic brain. Only neuron-specific labeling with the anti-hrp antibody is shown for omparison with (D).
(D) Lateral vie of an embryonic brain. Same preparation as in (C) but with both en and neuron-specific anti-HRP labeling shown. en-expressing cells in the brain appear as spots or clusters in the lateral view. Arrow, en-b1; arrowhead, en-b2; open arrow, en-b3.
(E) Frontal view of an embryonic brain. Only neuron-specific labeling with the anti-hrp antibody is shown for comnparison with (F). (F) Frontal view of an embryonic brain. Same preparation as in (E) but with both en and neuron-specific anti-HRP labeling shown. The two most anterior stripes of en-expressing cells in the brain are still detectable in this frontal view. en-b1 is indicated with arrows, en-b2 with arrowheads; en-b3 is not visible in this focal plane. The small groups of en-expressing cells anterior to en-b1 derive from the en-b1 stripe and move anteriorly ("secondary head spot" from Schmidt-Ott and Technau, 1992). A single en-expressing cell is seen at the midline of the preoral brain commissure.
Bar, 10.

Flybrain    Development    Embryonic Brain Development    Developmental Defects    Expression of Engrailed

All contents are copyright © 1995-2000 Flybrain or their original publication (as noted).
Copyright and use policy

AB00111