Expression of orthodentical in the Embryonic Brain

Laser confocal microscopy of wild-type stage 12 (A) and stage 16 (B and C) embryos double labeled with anti-otd antibody (yellow/green) and neuron-specific anti-HRP antibody (red/orange). Images are reconstructions of optical sections.

(A) Lateral view of otd-expression in the embryonic brain. Arrowheads, otd-expressing regions; open arrows, epidermal otd staining outside of the brain.
(B) Frontal view of otd expression in the embryonic brain. otd-expressing regions are delimited by arrowheads. Open arrow indicates immunostaining at the preoral brain commissure due to cross-reactivity of the anti-otd antibody with the glial specific repo/RK2 protein.
(C) Frontal view of otd expression in the embryonic brain. Same preparation as in (B), but sections were taken at a different focal plane. otd-expressing regions are delimited by arrowheads. Open arows indicate immunostaining at the medial edges of the descending brain tracts due to cross-reactivity as in (B).
Bar, 10.

Flybrain    Development    Embryonic Brain Development    Developmental Defects    Expression of orthodentical

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