The Embryonic Brain in otd Mutants (Reichert Lab)

The Embryonic Brain in otd Mutants

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Figure 4
The Embryonic Brain in otd Mutants.
Laser confocal microscopy of otd mutant stage 12 (A and B) and stage 13 (C and D) embryos labeled with neuron-specific anti-HRP antibody (red/orange; A and C), double labeled with anti-ems antibody (yellow/green) and neuron-specific anti-HRP antibody (red/orange; B), or double labeled with anti-repo/RK2 antibody (yellow/green) and neuron-specific anti-HRP antibody (red/orange; D). Images are reconstructions of optical sections.

(A) Lateral view of a mutant embryonic brain. Only neuron-specific labeling with the anti-hrp antibody is shown for comparison with (B). Asterisk, the missing brain neuromere b1.
(B) Lateral view of a mutant embryonic brain. Same preparation as in (A) but with both ems and neuron-specific anti-HRP lebeling shown. Arrow, ems expression in the neuromere b2; arrowhead, ems expression in the neuromere b3.
(C) Frontal view of a mutant embryonic brain. Only neuron-specific labeling with the anti-HRP antibody is shown for comparison with (D). The frontal nerve commissure of the stomatogastric nervous system is still present (open arrow). Arrowhead, missing preoral brain commissure; asterisks, missing brain neuromere b1.
(D) Frontal view of mutant embryonic brain. Same preparation as in (C) but with both anti-repo/RK2 and neuron-specific anti-HRP labeling shown. Open arrows, longitudinal glial cells associated with the embryonic circumesophageal connectives; asterisks, missing brain neuromere b1.
Bar, 10.

© Reichert Lab 1996

Flybrain Development Embryonic Brain Development Developmental Defects Embryonic Brain in otd Mutants

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