The Embryonic Brain in ems Mutants (Reichert Lab)

The Embryonic Brain in ems Mutants

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Figure 5
The Embryonic Brain in ems Mutants.
Laser confocal microscopy of ems mutant stage 12 (A and B) and stage 13 (C and D) embryos labeled with neuron-specific anti-HRP antibody (red/orange; A and C), double labeled with anti-en antibody (yellow/green) and neuron-specific anti-HRP antibody (red/orange; B), and double labeled with anti-repo/RK2 antibody (yellow/green) and neuron-specific anti-HRP antibody (red/orange; D). Images are reconstructions from optical sections.

(A) Lateral view of mutant embryonic brain. Only neuron-specific labeling with the anti-HRP antibody is shown for comparison with (B). Arrow, missing brain neuromeres b2 and b3.
(B) Lateral view of mutant embryonic brain. Same preparation as in (A) but with both enand neuron-specific anti-HRP labeling shown. Arrow indicates the en-b1 stripe viewed from lateral perspective that is still present in neuromere b1.
(C) Frontal view of mutant embryonic brain. Only neuron-specific labeling with the anti-HRP antibody is shown for comparison with (D). Arrowhead, preoral brain commissure; asterisks, missing brain neuromeres b2 and b3.
(D) Frontal view of mutant embryonic brain. Same preparation as in (C) but with both anti-repo/RK2 and neuron-specific anti-HRP labeling shown. Open arrows, midline glial cells associated with the preoral embryonic brain commissure; asterisks, missing brain neuromeres b2 and b3.
Bar, 10.

© Reichert Lab 1996

Flybrain Development Embryonic Brain Development Developmental Defects Embryonic Brain in ems Mutants

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